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- 详细信息
- 文献和实验
- 技术资料
- 库存：
34
- 英文名：
Docosahexaenoic Acid-d5 MaxSpec Standard
- CAS号：
1197205-71-2
- 供应商：
上海莼试
- 保存条件：
Store at -20°C
- 规格：
100μg
1.本蛋白酶抑制剂混合物为100×的储存液，使用时按照1:100的比例加入到裂解液中(例如，1ml裂解液中加入10μl蛋白酶抑制剂混合物)，混匀后即可使用。根据需要，0.5M的EDTA也按照1:100的比例加入到裂解液中（如用于检测金属蛋白酶活性，则不宜添加EDTA）。含有蛋白酶抑制剂混合物的裂解液宜现用现配，不宜配制后冻存待后续使用。
2. 待所需的抑制剂添加完毕混匀后，就可以开始进行哺乳动物组织的裂解和蛋白提取。
商品属性：
| 货号 | CS-01Y76064 | 规格 | 100μg |
| CAS号 | 1197205-71-2 | 分子量 | 333.5 |
| 含量 | >99.00% | 别名 | N/A |
| 分子式 | C22H27D5O2 | 化学名 | N/A |
| 产地 | 国产 | 用途 | 仅供科研研究实验 |
分子式:C22H27D5O2
分子量:333.5
溶解度:Soluble in DMSO
储存条件:Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request
Docosahexaenoic acid-d5 (DHA-d5) is intended for use as an internal standard for the quantification of DHA by GC- or LC-MS. DHA is an essential fatty acid and the most abundant ω-3 fatty acid in neural tissues, especially in the retina and brain.1 DHA constitutes as much as 40% of the total polyunsaturated fatty acid pool in retinal and neuronal membranes. Neonatal DHA deprivation leads to developmental defects and can lead to hypertension in rats.2 Docosahexaenoic acid-d5 MaxSpec standard is a quantitative grade standard of docosahexaenoic acid-d5 that has been prepared specifically for mass spectrometry and related applications where quantitative reproducibility is required. The solution has been prepared gravimetrically and is supplied in a deactivated glass ampule sealed under argon. The concentration was verified by comparison to an independently prepared calibration standard. This docosahexaenoic acid-d5 MaxSpec standard is guaranteed to meet identity, purity, stability, and concentration specifications and is provided with a batch-specific certificate of analysis. Ongoing stability testing is performed to ensure the concentration remains accurate throughout the shelf life of the product. Note: The amount of solution added to the vial is in excess of the listed amount. Therefore, it is necessary to accurately measure volumes for preparation of calibration standards. Follow recommended storage and handling conditions to maintain product quality.
注意事项：
抗逆滴加序列
每次向板内滴加抗原时，移液器滴头要与平面45度悬空，不要触碰到孔内的液体，由后向前依次滴加(即浓度由低往高滴加)。
抗感染反应期
抗原抗体在室温20~25℃下，必须反应30min以上，若环境温度低于室温，可将微量反应板置于恒温培养箱中，使二者充分反应。
旅游温度
磷酸盐缓冲液的pH值要在高压灭菌后进行滴定，往往在高压后pH值会有所改变，所以高压后再调一次pH值更为准确。磷酸盐缓冲液一经使用保存期不要超过3周。当pH<5.8时，红细胞会产生自凝现象;当pH>7.8时，图形洗脱加快，易造成肉眼观察产生误差;p H=7.2时，红细胞沉降最充分，图形最清晰。
当滴注 1%红细胞悬液时，应经常摇晃红细胞悬液，使红细胞均匀地分布在磷酸缓冲液中，以防止红细胞下降。
反应时间及温度
加入鸡红血球之后，反应板在室温(20~25℃)静置30~40min，对照孔血球下沉于孔底，即可判定结果。若室温达不到实验要求，需相应调整反应时间。当环境温度低于4℃时，红细胞发生自凝;高于37℃时，会发生反应物分离和红细胞溶血。
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文献和实验Tracking the Glycerophospholipid Distribution of Docosahexaenoic Acid by Shotgun Lipidomics
Docosahexaenoic acid (DHA, 22:6 n-3) is an omega-3 fatty acid with a 22 carbon acyl chain containing six cis double bonds and is predominantly found in membrane glycerophospholipids. Dietary consumption of DHA has been positively linked
Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry
macromolecules, including membrane lipids (2 ). Photoreceptor membranes in the retina contain an unusually high concentration of docosahexaenoic acid (22:6, omega 3), amounting to nearly 50% of the total fatty acid pool (3 ). By virtue of its structure, 22
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
25 mM Tris pH=8.0 1M 1,25 ml MPS2 for 10 ml 0,2 N NaOH 10N 200 ml 1% SDS 10% 1 ml H2O 8,8 ml MPS3 for 100 ml 5 M potassium acetate 60 ml glacial acetic acid 11,5 ml H2O 28,5ml
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