Thermo Scientific (Alfa Aesar)

SSR GEL and Silver Staining Protocol

the disposable filter unit. 2. Gels are 6% acrylamide, 8M urea, 1X TBE. For each gel, mix together 50 g urea, 15 ml 40% 19:1 acrylamide solution, and 31 ml d.i. water. We use a 4.5% gel for fingerprinting reactions. 3. Warm and stir the mixture

SSR GEL and Silver Staining Protocol

. 2. Gels are 6% acrylamide, 8M urea, 1X TBE. For each gel, mix together 50 g urea, 15 ml 40% 19:1 acrylamide solution, and 31 ml d.i. water. We use a 4.5% gel for fingerprinting reactions. Note: (We store aliquots of premixed 40% Acrylogel solution

【转贴】immunofluorescence staining

of use, make a 10% stock solution of the Triton X-100 in PEM buffer and aliquot to microcentrifuge tubes covered with foil. Store the stocks at -20 C.Coverslips:Round coverslips will fit in 24 well trays, and can be obtained from:Fisher Scientific, Cat