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文献和实验‐well microplate, low‐volume, flat bottom, non‐binding, black (Corning, cat. no. 3820) Matrix WellMate liquid dispenser (Thermo Scientific; Rudnicki and Johnston
Differential Display of Cotton Transcripts
) 25 mM MgCl2 alpha-[33P ]-dATP (NEN Research Products, du Pont de Nemours & Co, Boston, MA) 40% acrylamide:bisacrylamide (29:1) stock solution (Fisher Scientific, Houston, TX) TBE buffer: 0.09 M Tris-borate, 0.002
Immunohistochemisty-Enzymatic Protocol
# P6148) and 14 (v/v) saturated picric acid (Sigma Catalog # 925-40), pH 6.9. Picric acid is optional. Sucrose Solution: 130 mM Na2HPO4, 30 mM KH2PO4, 10 (w/v) sucrose (Sigma Catalog # S7903), 0.01 sodium azide (Sigma Catalog # S2002) and 0.03
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