Alexa Fluor® 647 Mouse Anti-Hu

AbC Anti-Mouse Bead Kit

together to calculate compensation in multicolor immunophenotyping experiments that incorporate a LIVE/DEAD® fixable dye by following the protocol outlined below: 1)        Gently vortex the ArC™ Amine Reactive Beads Kit and the AbC™ Anti-Mouse Bead Kit

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

【共享】常用抗体标记荧光染料的选择

的耐光性和良好的荧光量子产量著称。因此很多Alexa Fluor® 染料具有罗丹明核心结构，但是，传统罗丹明的化学结构很难从长波长的荧光染料延伸至远红外区域甚至是更具挑战性的近红外区域，而且生物偶联后其水溶性并不理想。Biotium 科学家发现从绿色到近红外多色荧光的罗丹明染料的新型化学方法。该方法被有效的应用于CF染料的产品中，尤其是远红外CF染料，而且通过这种方法制备的染料不仅水溶性极佳而且耐光性极好。图1. CF系列染料的稳定性，图示为CF633在5min中仍然具有稳定的荧光强度；而AF647