- ￥1078 - 7811
- abinScience 艾必赛生物
- JN967012
- 2026年07月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
广州市左克生物
- 规格：
50ug/100ug/1mg
| 规格： | 50ug | 产品价格： | ￥1078.0 |
|---|---|---|---|
| 规格： | 100ug | 产品价格： | ￥1494.0 |
| 规格： | 1mg | 产品价格： | ￥7811.0 |
| 货号 | JN967012 |
|---|---|
| 品牌 | abinScience |
| 描述 |
Recombinant CRM197 (Nontoxic Diphtheria Toxin), C-Strep-His (JN967012) expressed in E. coli, spanning Ala25-Ser560. Purity: >90% by SDS-PAGE.
Highlights
|
| 表达系统 | E. coli |
| Accession号 | P00587 |
| 蛋白长度 | Ala25-Ser560 |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 种属 | Corynephage omega |
| 性质 | Recombinant |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 预测分子量 | 62.87 kDa |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 重悬 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 别名 | Diphtheria toxin, DT, NAD(+)--diphthamide ADP-ribosyltransferase, Diphtheria toxin fragment A, Diphtheria toxin fragment B, CRM197, Nontoxic Diphtheria Toxin |
| 背景 |
Diphtheria toxin (CRM197 (Diphtheria toxin)) is a ~60 kDa protein. Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to elongation factor 2. Fragment A is responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A. |
| Note | For research use only. |
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octamers can be purified from native sources (Thomas & Butler, 1977) or assembled from recombinant histones (Luger et al, 1999). The latter method allows the incorporation of modified histones, or histone variants. Linker histones H1 and H5 isolated
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