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文献和实验(determine experimentally). A dilution series of 1:100 to 1:10,000 should cover it. Incubate at RT for 1 hr or longer, depending on the antibody. Sometimes an overnight incubation is helpful. Jon''s anti-actin Guinea pig antibodies from animal#1 are best
ELISA Procedure for Measuring Serum Antibody Titer
to the polystyrene microtiter plate first. The antiserum containing the anti-peptide antibody is then added to the well and allowed to bind. Finally, a second antibody, specific for the first antibody and labeled for detection, is added to the well and allowed
Yeast IF with Methanol/Acetone Dehydration
sufficient. Incubate in secondary antibody conjugate diluted in block solution for 1 hr at RT. You may need to determine the concentration best for your secondary antibody as well. The Cappel anti-Guinea Pig-FITC is best at 1:800-1:1,000. Wash
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