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文献和实验were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
Site-directed hydroxy radical mapping of nucleosome positions in vitro
for site directed mapping is BABE (bromoacetamidobenzyl EDTA (Meares et al ., 1984)). However, the predominant side reaction of common thiol-active functional groups such as bromoacetamide and maleimide is with lysine. There are a very large number
of the sample was incubated in 50 µl of terminal deoxynucleotidyltransferase (TdT) reaction mixture (Boehringer Mannheim; 200 mM potassium cacodylate, 25 mM Tris-HCl pH 6.6, 0.25 mg/ml bovine serum albumin, 1 mM CoCl2 , 0.25 nmol of FluoroLink Cy5AP3-dCTP
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