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文献和实验Western Blotting with Biotinylated Antibodies
) is now ready for loading onto your gel. For Protein Concentration Determination of Whole Tissue 1.Rapidly homogenize every 0.25g tissue in 3.5ml of boiling lysis buffer (1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 2.Microwave for 10-15 seconds
Western Blotting with Biotinylated Antibodies
Western Blotting with Biotinylated Antibodies SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline
Genome‐Wide Location Analysis by Pull Down of In Vivo Biotinylated Transcription Factors
the transcriptional regulator of interest with a short bio epitope which is specifically biotinylated by the E. coli enzyme BirA. The biotinylated transcription factor can then be selectively pulled down on streptavidin beads under stringent conditions. This unit
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