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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
- 英文名:
Recombinant Mouse UCP3 Protein, N-GST & C-His
- 库存:
999
- 供应商:
abinscience
- 规格:
50ug/100ug/1mg
| 规格: | 50ug | 产品价格: | ¥211.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥311.0 |
| 规格: | 1mg | 产品价格: | ¥1627.0 |

| Product name | Recombinant Mouse UCP3 Protein, N-GST & C-His |
|---|---|
| Catalog No. | MX158012 |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Purity | >90% as determined by SDS-PAGE. |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Endotoxin level | Please contact with the lab for this information. |
| Expression system | E. coli |
| Accession | P56501 |
| Protein length | Pro33-Ser73&Asp97-Arg116&Thr134-Trp179 |
| Nature | Recombinant |
| Predicted molecular weight | \ |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternative Names | Putative mitochondrial transporter UCP3, Slc25a9, Solute carrier family 25 member 9, UCP 3, Ucp3, Uncoupling protein 3 |
| Species | Mus musculus (Mouse) |
| Note | For research use only. |
Abinscience, founded in 2023 and located in the innovation technology center in Strasbourg, France, is the core research reagent brand of ProteoGenix. Focusing on the development and production of life science research reagents, Abinscience takes "Empowering Bioscience Discovery" as its vision, and is committed to providing high-quality and innovative biological reagent products and technical solutions for global researchers.
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文献和实验depends on product and its function. N-terminal sequencing revealed that the purified rBoNTC(Hc )-N is missing the first eight amino acids of the N-terminus of the protein, whereas the purified rBoNTC(Hc )-C protein is intact. After a mouse bioassay test
Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
总蛋白(包括质膜和细胞器膜蛋白),但费时费力。目前,有一种最新型有效的膜蛋白提取工具,并具有高纯度、高特异性、高载量温和提取的优点。是基于1980年科学家在牛眼视网膜中发现的一种命名为Rho1D4的氨基酸序列以及其对应的抗体来作为亲和标签纯化膜蛋白取得了非常好的效果,将Rho1D4抗体链接在琼脂糖或磁珠上,用于亲和层析带有Rho1D4标签的膜蛋白,能近乎完美的解决这些研究难题。1) 什么是Rho1D4?Rho1D4是指在牛眼视网膜细胞内的牛视紫红质C端的最后九位氨基酸。Rho1D4得名于与该序列
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