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- 抗体英文名：
Fixable Viability Stain 450
- 规格：
0.1mg
可用于固定破膜的检测方法，氨基反应染料，与细胞表面或者内部的游离胺不可逆的结合 .FVS系列光谱多样
BD Horizon
Application
Intracellular staining (flow cytometry), Flow cytometry (Tested During Development)
Description
BD Horizon™ Fixable Viability Stain 450 (FVS450) is useful to discriminate viable from non-viable mammalian cells in multicolor flow cytometric applications. This violet fluorescent stain contains a dye that reacts with and covalently binds to cell surface and intracellular amines. Permeable plasma cell membranes, such as those present in necrotic cells, allow for the intracellular diffusion of the violet dye and covalent binding to higher overall concentrations of amines than in non-permeable live cells. Therefore, necrotic cells present in a typical in vitro assay label with higher levels of dye increasing their fluorescence intensity 10-20 fold over that of viable cells. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeablization and subsequent intracellular staining while maintaining stable FVS450 fluorescence.
The BD Horizon™ Fixable Viability Stain 450 is excited by the Violet laser (with an excitation maximum of 406 nm) and has a fluorescence emission maximum at 450 nm.
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文献和实验Exercise 12.7 - Viability Cell Count Materials Suspension culture of cells Sterile transfer pipettes Stock 0.2% (w/v) Trypan blue Hemacytometer and microscope Procedure Gently
Trypan blue will stain dead or dying cells. Viable cells are able to repell the dye and do not stain. Note: Trypan blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted
Viability Cell Count Materials Suspension culture of cells Sterile transfer pipettes Stock 0.2% (w/v) Trypan blue Hemacytometer and microscope Procedure Gently
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