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- fermentas
- B34
- 2025年09月05日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
4 x 1.25 mL
描述
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.10X Taq Buffer with (NH4)2SO4 and 20 mM MgCl2 (B34) includes:
• 750 mM Tris-HCl (pH 8.8 at 25°C)
• 200 mM (NH4)2SO4
• 0.1% (v/v) Tween 20
• 20 mM MgCl2
Related Products
Taq Buffer (10X), without detergent
Taq Buffer (10X), with KCl and 15 mM MgCl2
Taq Buffer (10X), with KCl
Taq Buffer with (NH4)2SO4 (10X)
规格
| Polymerase: | Taq DNA Polymerase |
|---|---|
| Product Size: | 4 x 1.25 mL |
内容及储存
• 4 x 1.25 mL 10X Taq Buffer with (NH4)2SO4 and 20 mM MgCl2 Store at -20°C.
规格
| Polymerase: | Taq DNA Polymerase |
|---|---|
| Product Size: | 4 x 1.25 mL |
内容及储存
• 4 x 1.25 mL 10X Taq Buffer with (NH4)2SO4 and 20 mM MgCl2 Store at -20°C.
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文献和实验2 concentration is 1-4mM, under the standard reaction conditions specified. In our experiments, at a final dNTP concentration of 0.2mM, a MgCl2 concentration ranges of 1.5±0.25mM (in traditional PCR buffer) and of 2.0±0.5mM (in PCR buffer with (NH4)2SO4
Mouse p27 PCR Using Gitschier Buffer
)2 SO4 166mM 33.5 mL 1 M Tris base pH 8.8 670 mM 174 µL ß -Mercaptoethanol 50 mM 3.35 mL 1M MgCl 2 67 mM q.s. to 50 mL
Table 1 Components Volume Final Concentration 10X PCR Buffer, Minus Mg
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