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300 units
描述
Thermo Scientific T7 DNA Polymerase, a template dependent DNA polymerase, catalyzes DNA synthesis in the 5'→3' direction. It is a highly processive DNA polymerase allowing continuous synthesis of long stretches of DNA. The enzyme also exhibits a high 3'→5' exonuclease activity towards single- and double-stranded DNA.Highlights
• Strong 3'→5' exonuclease activity, approximately 1000-fold greater than Klenow Fragment
• Active in restriction enzyme buffers
Applications
• Purification of covalently closed circular DNA by removal of residual genomic DNA
• Primer extension reactions on long templates (see Reference 1)
规格
| Polymerase: | T7 DNA Polymerase |
|---|---|
| Product Size: | 300 units |
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文献和实验mRNA Amplification with T7 RNA Polymerase
on ice. Second-strand cDNA synthesis Set PCR machine to hold at 16ºC. On ice prepare 2nd Strand Master Mix (per sample): 63 µL Nuclease-free H2O 10 µL 10x 2nd strand buffer 4 µL dNTP mix 2 µL DNA polymerase 1 µL
In Situ Labeling of DNA Breaks and Apoptosis by T7 DNA Polymerase
The native T7 DNA polymerase is a fast and highly processive enzyme that can be used for in situ detection of apoptosis and various types of DNA breaks. The technique is quick and simple, and was shown to label earlier stages
DNA Sequencing Using Sequenase Version 2.0 T7 DNA Polymerase
The dideoxy chain-termination method of DNA sequence analysis involves the synthesis of a DNA strand by enzymatic extension from a specific primer using a DNA polymerase (1 ). Several different enzymes are available for this purpose, each having
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