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- AF0016
- 2025年09月05日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
磷酸化Akt抗体，(Ser473)
- 抗原来源：
人，大小鼠
- 是否单克隆：
多克隆
- 抗体英文名：
Phospho-Akt(Ser473) Antibody
- 规格：
50ul
Phospho-Akt(Ser473) Antibody
名 称:
磷酸化 Akt(Ser473) 抗体
货 号:
AF0016
来 源:
Rabbit
应 用:
WB,IHC,IF/ICC,ELISA
反 应:
Human, Mouse, Rat
预 测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
蛋白号:
P31749P31751Q9Y243
分子量:
60kDa
浓 度:
1mg/ml
Alternative Names:Expand▼
AKT 1; AKT; AKT1; AKT1_HUMAN; MGC99656; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; Akt2; AKT2_HUMAN; HIHGHH; murine thymoma viral (v-akt) homolog-2; PKB; PKB beta; PKBB; PKBBETA; PRKBB; Protein kinase Akt 2; Protein kinase Akt-2; Protein kinase B beta; rac protein kinase beta; RAC-BETA; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v akt murine thymoma viral oncogene homolog 2; Akt3; AKT3 kinase; AKT3_HUMAN; DKFZp434N0250; MPPH; PKB gamma; PKBG; PRKBG; Protein kinase Akt-3; Protein Kinase AKT3; Protein kinase B gamma; RAC gamma; RAC gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; RACPK Gamma; Serine threonine protein kinase Akt 3; Serine threonine protein kinase Akt3; STK 2; STK-2; STK2; V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); V akt murine thymoma viral oncogene homolog 3; V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma;
Applications:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Reactivity:
Human, Mouse, Rat
Predicted Reactivity:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Source:
Rabbit
Clonality:
Polyclonal
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Specificity:
Phospho-Akt(Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Sersine 473.
Format:
Liquid
Concentration:
1mg/ml
文献：Reference Citations:
Application: WB Species:human; Sample:Not available
Fig. 4 Fucoxanthin promotes apoptosis via inhibition of PI3K/Akt/ mTOR signaling pathway in glioblastoma cells. a Cell lysates were electrophoresed and Akt, p-Akt, mTOR and p-mTOR proteins were detected by their respective specific antibodies in indicated concentrations
Application: WB Species:human; Sample:Not available
Treatment with angelicin alters the protein expression levels of PI3K, p-Akt and total Akt in HepG2 and Huh-7 cells in vitro. (A) Representative blots demonstrating PI3K, p-Akt and total Akt protein expression levels in HepG2 and Huh-7 cells, following treatment with various concentrations of angelicin. (B) PI3K blots were semi-quantified using densitometry analysis of the protein bands and normalized to GAPDH. (C) p-Akt/Akt blots were semi-quantified using densitometry analysis of the protein bands. (D) Effects of LY294002 on angelicin-induced apoptosis. Cells were treated with the PI3K inhibitor LY294002 (3 mM) for 1 h prior to treatment with angelicin. The percentage of apoptotic cells following treatment with angelicin in the presence or absence of LY294002 was assessed using Annexin V-fluorescein isothiocyanate/propidium iodide staining and flow cytometry. Data are expressed as the mean ± standard deviation of 3 independent experiments.
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文献和实验(Ser473)(193H12)Rabbit mAb，1:300,稀释，二抗是驴抗兔，1：2000，我是实验室的新手，刚呆俩月，受 此挫折，一头雾水，请过来人或者有经验的同学帮忙解答怎样解决，非常感谢！ 碧峤 pAkt有两个位点，Ser473和Thr308，其中Ser473相对容易显色。如果显色失败，可能的原因:1 上样量过少，我们一般都是30μg；2 一抗孵育时间过短，我们一般24h~48h；3 一抗是否工作有待排除 米宝
receptorr 我在做HaCaT细胞的磷酸化Akt(473)时，背景比较高，该怎么办？（即未处理组的Akt比较高） 请高手支招。 谢谢 magichunter 说得太粗略了。 receptorr 我做的细胞是HaCaT 看看加入药物后HaCaT的磷酸化Akt是否有增高， 所以希望没有加药的那一组HaCaT的磷酸化Akt水平低 但是实际做时，发现没有
全相同. 不管是总量变化, 还是磷酸化变化, 都能够进行讨论 赞同！ 楼主还是要多看文献参考参考才行。 通吃 我觉着那要看你的研究目的了吧 只有完全磷酸化了的Akt，即Ser473 Thr308位点都磷酸化之后，Akt激酶的活性才能充分发挥的。 doctormy licanming wrote: 有区别的！要看你观察的实验指标与磷酸化哪个残基
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