Quick CIP，[NEB M0525S]

Quick CIP is a heat-labile version of calf intestinal alkaline phosphatase (CIP) purified from a recombinant source.

• Rapid and irreversible heat inactivation eliminates unwanted activity
• Improved storage stability versus native enzyme
• Faster reaction setup (no supplemental additives like zinc required) and shorter incubation time
• Flexible reaction conditions (active in any restriction enzyme buffer, no clean-up required)
• Less enzyme required (high specific activity), resulting in a lower cost per reaction
• No need for multiple phosphatases (Quick CIP removes 5′- and 3′- phosphates from DNA, RNA and dNTPs )
• Active on unincorporated dNTPs in PCR products - improves DNA sequencing and SNP analysis
• Recombinant for purity, consistency and value

• 产品组分信息

  本产品提供以下试剂或组分：

  NEB #	名称	组分货号	储存温度	数量	浓度
  M0525V     -20       Quick CIP M0525VVIAL -20 1 x 0.1 ml 5,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  M0525S     -20       Quick CIP M0525SVIAL -20 1 x 0.2 ml 5,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  M0525L     -20       Quick CIP M0525LVIAL -20 1 x 1 ml 5,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X

  Quick CIP是从重组来源纯化的小牛肠道碱性磷酸酶（CIP）的热不稳定重组版本。Quick CIP非特异性催化DNA和RNA磷酸单酯5′端和3′端的去磷酸化。它还水解核糖核苷和脱氧核糖核苷三磷酸（NTP和dNTP）。快速CIP在许多需要DNA或RNA末端去磷酸化的应用中是有用的。在克隆过程中，去磷酸化可以防止线性化质粒DNA的重新连接。该酶可以在10分钟内快速对突出的5′、凹陷的5′和钝端脱磷。快速CIP也可用于降解PCR反应中未合并的dNTP，以制备用于DNA测序或SNP分析的模板。
  
  
  快速CIP通过在80°C下加热2分钟而完全不可逆地失活，而野生型CIP不可热失活。这使得在结扎或末端标记之前去除Quick CIP是不必要的。
  
  产品来源
  
  一种毕赤酵母克隆，携带来自小牛肠粘膜的小牛肠碱性磷酸酶基因。