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- 2025年08月28日
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PE Rat IgG2c, κ Isotype Control
- 规格：
0.1mg
BD Pharmingen
Concentration
0.2 mg/ml
Isotype
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2c, κ
Application
Flow cytometry, Isotype control (Routinely Tested)
Immunogen
TNP-keyhole limpet hemocyanin
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
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