The eBioscience ELISA/ELISPOT Coating Buffer Powder is designed and
tested for use as the working solution for coating the ELISA or ELISPOT
capture antibody to the ELISA or ELISPOT plate microwells. This has
been tested with all cytokine ELISA and ELISPOT capture antibodies,
according to the procedures described in the corresponding protocols.
The eBioscience ELISA/ELISPOT Coating Buffer Powder is a dried powder
formulation of phosphate buffered saline. Each packet is reconstituted
in 1 Liter of distilled, deionized water, then filtered using a 0.22
um filter. The resulting 1x PBS (pH 7.4) is suitable for use as the
ELISA/ELISPOT coating buffer.