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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
兔
- 应用范围:
WB IHC
- 适应物种:
人 大鼠 小鼠
- 是否单克隆:
多克隆
- 抗体英文名:
Cleaved-caspase 3 antibody
- 规格:
50ug
Catalog No. : AF7022
Size : 50μg/50μl, 100μg/100μl
Mol Weight : 17kDa
Source : Rabbit
Clonality : Polyclonal
Alternative Names:
Apopain precursor; CASP-3; CPP-32; CPP32; Caspase-3; Cysteine protease CPP32; ICE3; SCA-1; SREBP cleavage activity 1; Yama protein,p17
Applications:
WB 1:500-1:2000 IHC 1:50-1:200
Reactivity:
Human Mouse Rat
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Purification:
Immunogen affinity purified
Specificity:
Caspase 3 (p17,Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Caspase 3.
Description:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 13 months from date of receipt
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文献和实验Neoepitope Antibodies Against MMP-Cleaved and Aggrecanase-Cleaved Aggrecan
in studies determining the contribution of matrix metalloproteinases (MMPs) and aggrecanases to aggrecanolysis. In particular, an antibody to the A374 RGSV N terminus was instrumental in the landmark discovery of the aggrecanases, ADAMTS-4 and ADAMTS-5
或 C6 cells 作为 cleaved caspase-3 的阳性参照。更多靶点的阳性对照,请登陆CST网站——研究——靶标的阳性对照处理。图二 蛋白质印迹分析(图源自CST Caspase-3 Antibody #9662 抗体说明书)为什么所有的实验都要设置对照?无论是初高中刚接触生物学实验设计还是上研究生后发表 SCI 文章,老师们以及 reviewer 都会敲着「黑板」强调强调再强调,每一个实验都必须有对照!因为设置适当的阴性、阳性甚至空白对照对于实验结果的准确、有效阐释至关重要!阅历丰富
【求助】请问western blot做凋亡检测时,识别caspase-3酶原和激活的caspase-3的抗体是相同的吗?
lsdcfhyj 以及检测PARP的裂解前后蛋白水平用的抗体是否都是一样的? edwardellen 有两者都识别的,也有专门识别剪切体不识别酶原的,用过cell signal的,两种都很好用。 PARP也有可以同时识别全长和剪切体的抗体。 magichunter 检测caspase-3和激活型的caspase-3分别需要两种不同的抗体,一般CST公司卖的分别包括:cleaved
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