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- abcam
- ab182141
- 2025年10月27日
- WB, IHC-P, ICC/IF
- 兔
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 靶点：
Prostaglandin D Synthase (Lipocalin)/PDS
- 级别：
PTGDS
- 目录编号：
ab182141
- 保质期：
Prostaglandin D Synthase (Lipocalin)/PDS
- 抗体英文名：
Prostaglandin D Synthase (Lipocalin)/PDS抗体
- 抗体名：
Prostaglandin D Synthase (Lipocalin)/PDS
- 宿主：
兔
- 适应物种：
人
- 应用范围：
WB, IHC-P, ICC/IF
- 浓度：
Prostaglandin D Synthase (Lipocalin)/PDS抗体
- 保存条件：
低温
- 规格：
10ul
重组Anti-Prostaglandin D Synthase (Lipocalin)/PDS抗体[EP12357] (ab182141)
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
-
描述
兔单克隆抗体[EP12357] to Prostaglandin D Synthase (Lipocalin)/PDS -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab188544) -
阳性对照
- HepG2 whole cell lysate (ab7900); Human fetal brain lysate; Human prostate tissue; HepG2 cells.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
-
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
浓度
- 100 µl 浓度为 2.287 mg/ml
- 40 µl 浓度为 2.287 mg/ml
- 10 µl 浓度为 2.287 mg/ml
应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
应用 Ab评论 说明 WB 1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 21 kDa).Can be blocked with Prostaglandin D Synthase (Lipocalin)/PDS peptide (ab188544).IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.ICC/IF 1/100.
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文献和实验的底物进行筛选。我们通过离体印迹磷酸化作用来确认可能的底物。总的来说，我们的方法能筛选植物蛋白激酶用于进一步的分析，随后的体内实验对评估它们的生理作用是必不可少的 [ 13，24，25 ]。2. 材料 2.1 非变性条件下重组激酶的纯化 ( 1 ) 培养细胞的培养基：含 100 μg/ml 氨苄青霉素，15 μg/ml 卡那霉素，2% 葡萄糖的 2YB 或 LB 培养基。 ( 2 ) 异丙基 β-D-硫代半乳糖苷（IPTG ) 。 ( 3 ) 非变性裂解液：300 mmol
Liposomal Reconstitution of Monotopic Integral Membrane Proteins
is required for establishing several aspects of the functions of membrane proteins and lipids and for elaborating models of naturally occurring membranes. Cyclooxygenase (COX)-1 and -2 (also prostaglandin endoperoxide H2 synthase, PGHS
Naito, S. and Shimamoto, K. (2000) Post-transcriptional gene silencing in cultured rice cells.Plant Cell Physiol. 4 1 , 321 -3 26 . 3. F ire, A ., X u , S. Q ., M ontgomery, M. K ., K o stas, S. A ., D river, S. E. and
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