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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Alexa Fluor® 647 Mouse anti-PLCγ1
- 规格：
50t
BD Phosflow
Vol. Per Test
20 µl
Isotype
Mouse IgG1
Reactivity
Human (QC Testing) Mouse, Rat, Dog, Chicken (Tested in Development)
Application
Intracellular staining (flow cytometry) (Routinely Tested)
Immunogen
Cow PLCγ1 N-terminal region Peptide
Description
The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLCγ is the only member that contains SH2 and SH3 domains. These domains enable it to interact with receptor tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: 1) PLCγ1, which is ubiquitously expressed, and 2) PLCγ2, found primarily in the lymphoid system. PLCγ is essential for growth factor-induced cell motility and mitogenesis. PLCγ1 null mice exhibit retarded embryonic growth and lethality in midgestation. Overexpression of PLCγ is evident in several forms of cancer, and it has been identified as a key mediator of PDGF-dependent cellular transformation. Thus regulation of PLCγ activity by growth factors is involved in cell growth and transformation.
The 10/PLCgamma monoclonal antibody recognizes PLCγ1, regardless of phosphorylation status. It does not cross-react with PLCγ2.
Format
- Format
- Alexa Fluor® 647
- Excitation Source
- Red 633 nm
- Excitation Max
- 650 nm
- Emission Max
- 668 nm
Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.
Suggested Companion Products
| Cat No. | Description | Size | |
|---|---|---|---|
| 558050 | Perm Buffer III RUO | 125 mL |
|
| 554655 | Fixation Buffer RUO | 100 mL |
|
| 554656 | Stain Buffer (FBS) RUO | 500 mL |
|
| 554657 | Stain Buffer (BSA) RUO | 500 mL |
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文献和实验的耐光性和良好的荧光量子产量著称。因此很多Alexa Fluor® 染料具有罗丹明核心结构，但是，传统罗丹明的化学结构很难从长波长的荧光染料延伸至远红外区域甚至是更具挑战性的近红外区域，而且生物偶联后其水溶性并不理想。Biotium 科学家发现从绿色到近红外多色荧光的罗丹明染料的新型化学方法。该方法被有效的应用于CF染料的产品中，尤其是远红外CF染料，而且通过这种方法制备的染料不仅水溶性极佳而且耐光性极好。图1. CF系列染料的稳定性，图示为CF633在5min中仍然具有稳定的荧光强度；而AF647
技术原理与方法｜超级色差校正物镜 PLAPON60XOSC 在脑组织四重免疫荧光分析中的应用
种类型的荧光抗体在单个标本中准确展示荧光标记分子的共定位情况。 PLAPON60XOSC 和 UPLSAPO60XO 的性能比较 2. 超级色差校正物镜的应用-脑组织的四重免疫荧光 图 1. 脑组织的四重免疫荧光。 VIAAT 染色（Alexa Fluor405，蓝色）；CB1 染色（Alexa Fluor488，绿色）；VGluT3 染色（Cy3，红色）；DGLα染色（Alexa Fluor647，白色）。 大麻素合成酶 DGLα（白色）和大麻素受体 CB1（绿色）聚集在小鼠
'）2 Fragment（Alexa Fluor® 555 Conjugate）#4413 Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 647 Conjugate）#4414 抗小鼠： Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4408 Anti-Mouse IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor
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