- ￥3356
- OriGene
- 美国
- TA398076
- 2026年04月01日
- ELISA, IHC, WB
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Donkey IgG whole molecule
- 形态：
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
- 保存条件：
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 克隆性：
Polyclonal
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA398076
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Goat
- 应用范围：
ELISA, IHC, WB
- 抗体英文名：
Donkey IgG (H&L) Antibody Alkaline Phosphatase Conjugated
- 抗体名：
Donkey IgG (H&L) Antibody Alkaline Phosphatase Conjugated
- 规格：
1 mg
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
. DO NOT DIFFERENTIATE IN ACID ALCOHOL. 26. Mount in an AQUEOUS MOUNTANT. Results --Nuclei Blue --Sites of alkaline phosphatase activity Red Substrate Solution Naphthol-AS-MX phosphatase, free acid (Sigma N 4875) 2 mg N,N-Dimethyl formamide
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
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