- ￥3938
- OriGene
- 美国
- TA397704
- 2026年04月01日
- ELISA, IHC, WB
- Goat
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- 文献和实验
- 技术资料
- 免疫原：
Horse IgG F(ab')2 fragment
- 形态：
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
- 保存条件：
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 克隆性：
Polyclonal
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA397704
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Goat
- 应用范围：
ELISA, IHC, WB
- 抗体英文名：
Horse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
- 抗体名：
Horse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
- 规格：
1 mg
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of X-gal can be easier to detect prior to carrying out the X-Phos reaction as background alkaline phosphatase staining can obscure it, particularly in whole mounts. Rinse the tissue very well with PBS prior to PLAP staining as residual ß-gal activity
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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