- ￥1999
- OriGene
- 美国
- TA814282
- 2026年04月01日
- IHC
- Mouse
- Human
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- 文献和实验
- 技术资料
- 免疫原：
Human recombinant protein fragment of human Nestin(NES) (NP_006608) produced in E.coli.
- 形态：
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
- 保存条件：
Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA814282
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
IHC
- 靶点：
Nestin
- 抗体英文名：
Nestin(NES) mouse monoclonal antibody,clone OTI8C4
- 抗体名：
Nestin(NES) mouse monoclonal antibody,clone OTI8C4
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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