- ￥3939
- OriGene
- 美国
- AM39061SU-N
- 2026年04月01日
- IF, IHC
- Mouse
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
State: SupernatantState: Liquid cell culture supernatant containing 0.09% sodium azide
- 保存条件：
Store undiluted at 2-8°C.
- 克隆性：
Monoclonal
- 适应物种：
Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM39061SU-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
IF, IHC
- 抗体英文名：
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant
- 抗体名：
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone HIS40, Supernatant
- 规格：
1 ml
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
information for VH and VL is generally amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody-specific primers A variety of primer sets for the amplification of mouse-variable domams has been developed that are particulary
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