- ￥3999
- OriGene
- CF811690
- 美国
- 2026年04月01日
- WB
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Carrier-free (BSA/glycerol-free) E2F4 mouse monoclonal antibody,clone OTI10E7
- 抗体英文名：
Carrier-free (BSA/glycerol-free) E2F4 mouse monoclonal antibody,clone OTI10E7
- 靶点：
E2F4
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
CF811690
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原：
Human recombinant protein fragment corresponding to amino acids 86-413 of human E2F4 (NP_001941) produced in E.coli.
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production Protocol
for 5 - 10 min. Aspirate the medium, resuspend pellet and wash again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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