- ￥2350
- OriGene
- TA346872
- 美国
- 2026年04月01日
- FC, ICC, IF, WB
- Mouse
- Human, Monkey, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Mouse Monoclonal ATP-Citrate Lyase (C-terminus) Antibody
- 抗体英文名：
Mouse Monoclonal ATP-Citrate Lyase (C-terminus) Antibody
- 靶点：
ATP citrate lyase
- 应用范围：
FC, ICC, IF, WB
- 宿主：
Mouse
- 适应物种：
Human, Monkey, Mouse
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA346872
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
- 免疫原：
The immunogen for ACLY antibody: purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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