- ￥2350
- OriGene
- TA346963
- 美国
- 2026年04月01日
- IP, WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Mouse Monoclonal HDAC4 (N-terminus) Antibody
- 抗体英文名：
Mouse Monoclonal HDAC4 (N-terminus) Antibody
- 靶点：
HDAC4
- 应用范围：
IP, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
TA346963
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
- 免疫原：
The immunogen for HDAC4 antibody: purified recombinant human HDAC4 protein fragments expressed in E.coli
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Production of Monoclonal Antibody by DNA Immunization with Electroporation
against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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