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- 文献和实验
- 技术资料
- 抗体名:
Mouse Monoclonal Aldehyde dehydrogenase 10 Antibody
- 抗体英文名:
Mouse Monoclonal Aldehyde dehydrogenase 10 Antibody
- 靶点:
Aldehyde dehydrogenase 10
- 应用范围:
WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA346881
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
- 免疫原:
The immunogen for ALDH3A2 antibody: purified recombinant human Aldehyde dehydrogenase 10 protein fragments expressed in E.coli.
- 规格:
100 ul
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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