- ￥3999
- OriGene
- CF810618
- 美国
- 2026年04月01日
- WB
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Carrier-free (BSA/glycerol-free) BAG5 mouse monoclonal antibody,clone OTI3C10
- 抗体英文名：
Carrier-free (BSA/glycerol-free) BAG5 mouse monoclonal antibody,clone OTI3C10
- 靶点：
BAG5
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
CF810618
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原：
Full length human recombinant protein of human BAG5 (NP_004864) produced in 293T.
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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