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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Carrier-free (glycerol/BSA-free) Acidic CK rat monoclonal antibody, clone OTI9H3 (formerly 9H3)
- 抗体英文名:
Carrier-free (glycerol/BSA-free) Acidic CK rat monoclonal antibody, clone OTI9H3 (formerly 9H3)
- 靶点:
Acidic CK
- 应用范围:
IHC
- 宿主:
Rat
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
CF190248
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原:
Synthetic peptide corresponding to the conserved region of human type I (Acidic) keratins (including KRT9, KRT10, KRT12, KRT13, KRT14, KRT15, KRT16, KRT17, KRT18, KRT19, KRT20).
- 规格:
100 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
Large-scale immunofluorescence
overnight7 at 4o C with very gentle shaking. Wash cells once with PBS, once with PBS plus 0.1% NP40, and then again with PBS. Resuspend cells in 30 ul of PBS plus 0.1% BSA containing the secondary antibody8 , and incubate for 4 hours at room
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