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- 文献和实验
- 技术资料
- 抗体名:
Carrier-free (BSA/glycerol-free) Troponin C (TNNC1) mouse monoclonal antibody,clone UMAB204
- 抗体英文名:
Carrier-free (BSA/glycerol-free) Troponin C (TNNC1) mouse monoclonal antibody,clone UMAB204
- 靶点:
Troponin C1
- 应用范围:
10k-ChIP, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
UM800096CF
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原:
Full length human recombinant protein of human TNNC1 (NP_003271) produced in E.coli.
- 规格:
100 ug
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文献和实验Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
Monoclonal Antibody Production Protocol
buffer. (ex: HRP is 2000X). Leave 1 hr at room temp or O.N at 4°C. SUBSTRATE Dissolve substrate in water. Wash plate 4 times with PBS + 0.1% Tween 20 (use TBS instead of PBS for AP). Add 100 ul of substrate to every well. Match color development
Western Blotting with Monoclonal Ant
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. Polyacrylamide Gel
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