- ￥4999
- OriGene
- UM800102CF
- 美国
- 2026年04月01日
- 10k-ChIP, FC, IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody,clone UMAB210
- 抗体英文名：
Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody,clone UMAB210
- 靶点：
LGR5
- 应用范围：
10k-ChIP, FC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
UM800102CF
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原：
Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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