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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Carrier-free (BSA/glycerol-free) Purified HER2 (ERBB2) mouse monoclonal antibody,clone UMAB35
- 抗体英文名:
Carrier-free (BSA/glycerol-free) Purified HER2 (ERBB2) mouse monoclonal antibody,clone UMAB35
- 靶点:
Her2
- 应用范围:
10k-ChIP, IF, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human, Dog, Mouse, Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
UM500035CF
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原:
Human recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
- 规格:
100 ug
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文献和实验Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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