- ￥3999
- OriGene
- CF807333
- 美国
- 2026年04月01日
- IHC
- Mouse
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Carrier-free (BSA/glycerol-free) PISD mouse monoclonal antibody,clone OTI6C3
- 抗体英文名：
Carrier-free (BSA/glycerol-free) PISD mouse monoclonal antibody,clone OTI6C3
- 靶点：
PISD
- 应用范围：
IHC
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
CF807333
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原：
Human recombinant protein fragment corresponding to amino acids 80-340 of human PISD(NP_055153) produced in E.coli.
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
-galactopyranoside (X-gal; 25 mg/mL in DMF [dimethylformamide]) Agarose gel (1%, UltraPure; Invitrogen) Antibody, specific for the protein of interest Bead eluting buffer Bovine serum albumin (BSA; 0.5% in PBS; New England Biolabs) Cell
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