- ￥5918
- OriGene
- AM06200SU-N
- 美国
- 2026年04月01日
- IHC, WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
HCK mouse monoclonal antibody, clone 3D12E10, Ascites
- 抗体英文名：
HCK mouse monoclonal antibody, clone 3D12E10, Ascites
- 靶点：
HCK
- 应用范围：
IHC, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM06200SU-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态：
State: AscitesState: Ascitic fluid containing 0.03% Sodium Azide.
- 免疫原：
Purified recombinant fragment of HCK expressed in E. Coli.
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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