- ￥4136
- OriGene
- AM08052PU-N
- 美国
- 2026年04月01日
- FC, FN, IP
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Cd80 rat monoclonal antibody, clone 1G10, Purified
- 抗体英文名：
Cd80 rat monoclonal antibody, clone 1G10, Purified
- 靶点：
Cd80
- 应用范围：
FC, FN, IP
- 宿主：
Rat
- 适应物种：
Mouse
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM08052PU-N
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态：
100 mM Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.State: PurifiedState: Liquid purified Ig fraction.
- 规格：
500 ug
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文献和实验Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
问：BAF cell的来源是什么？有什么特性和用途呢？答：ATCC上没有你所谓的BAF cell，DSMZ上有两种BAF cell，如下：Cell line:BA-F8Cell type:mouse hybridoma(anti-rat fiber/myosin)Origin:this hybridoma cell line was described to secrete a mouse monoclonal antibody(IgG2b)that reacts with rat type
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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