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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
C5R1 (C5AR1) rat monoclonal antibody, clone 8D6, PE
- 抗体英文名:
C5R1 (C5AR1) rat monoclonal antibody, clone 8D6, PE
- 靶点:
C5R1
- 应用范围:
FC
- 宿主:
Rat
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM31865RP-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store the antibody undiluted at 2-8°C.DO NOT FREEZE!
- 形态:
PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.Label: PEState: Liquid purified Ig fractionLabel: R - Phycoerythrin
- 免疫原:
RBL-2H3 (rat basophilic leukemia) transfected cells expressing human C5aR on the cell surfaceDonor: Lou/c rat spleen cells
- 规格:
50 ug
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
, 0.1mM ßME. plus 3 U/mL Epo, 50 ng/mL Tpo, 100 ng/mL SCF, 100 ng/mL IL-6, ? ng/mL IL-3. Antibodies for Lineage Depletion: (Pharmingen) Antibodies for Sorting: (Pharmingen monoclonal rat anti-mouse)
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