- ￥5346
- OriGene
- AM06196SU-N
- 美国
- 2026年04月01日
- WB
- Mouse
- Horseradish Peroxidase
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- 文献和实验
- 技术资料
- 抗体名：
Horseradish Peroxidase / HRP mouse monoclonal antibody, clone 3A5C6, Ascites
- 抗体英文名：
Horseradish Peroxidase / HRP mouse monoclonal antibody, clone 3A5C6, Ascites
- 应用范围：
WB
- 宿主：
Mouse
- 适应物种：
Horseradish Peroxidase
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM06196SU-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态：
State: AscitesState: Ascitic fluid containing 0.03% Sodium Azide.
- 免疫原：
Purified recombinant fragment of HRP expressed in E. Coli.
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Polyclonal and Monoclonal Antibodies
). Enzyme linked antibody = Horseradish peroxidase 1 Substrate = ABTS - 100X (from Zymed 00-2001)1 HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dihydrate + 50 ml H2O, pH 4.2 ). Hydrogen peroxide 30
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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