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- 文献和实验
- 技术资料
- 抗体名:
Prostatic Acid Phosphatase (ACPP) rabbit polyclonal antibody, Azide Free
- 抗体英文名:
Prostatic Acid Phosphatase (ACPP) rabbit polyclonal antibody, Azide Free
- 靶点:
Prostatic Acid Phosphatase
- 应用范围:
ELISA, ID, IF, IHC, IP, R, WB
- 宿主:
Rabbit
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AP21364AF-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 克隆性:
Polyclonal
- 保存条件:
Prior to reconstitution store at 2-8°C.Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态:
PBS, pH 7.2 without preservatives and foreign proteins.State: Azide FreeState: Lyophilized hyperimmune IgG fraction
- 免疫原:
Acid Phosphatase isolated and purified from Human seminal plasma.Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 规格:
10 mg
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文献和实验ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
antibody. 30 minutes. 5. Jet wash in tris/HC1 saline buffer pH 7.6. 6. Wash in buffer. 3 minutes. Steps 7-9 for POLYCLONAL Primary Antisera Only 7. Incubate in mouse anti-rabbit immunoglobulins. 30 minutes. 8. Jet wash in buffer.
Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
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