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- 文献和实验
- 技术资料
- 抗体名:
Heparanase 1 (HPSE) rabbit polyclonal antibody, Purified
- 抗体英文名:
Heparanase 1 (HPSE) rabbit polyclonal antibody, Purified
- 靶点:
HPSE
- 应用范围:
ELISA, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AP54993PU-N
- 级别:
科研级
- 库存:
8周
- 供应商:
OriGene
- 克隆性:
Polyclonal
- 保存条件:
Prior to reconstitution store at -20°C.Following reconstitution store undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态:
0.1M Tris, 0.1M Glycine, 2% SucroseState: PurifiedState: Lyophilized purified powder
- 免疫原:
Synthetic peptide derived from Human N-terminal region of Heparanase (HPSE)
- 规格:
100 ug
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文献和实验Use of EIA to Measure MMPs and TIMPs
the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal
Tips and hints for the storage of antibodies
! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at ℃. Serum does not contain active proteases
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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