- ￥5346
- OriGene
- AM06387SU-N
- 美国
- 2026年04月01日
- ELISA, FC, IF, IHC, WB
- Mouse
- Human
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- 文献和实验
- 技术资料
- 抗体名：
Cytokeratin 15 (KRT15) mouse monoclonal antibody, clone 6E7, Ascites
- 抗体英文名：
Cytokeratin 15 (KRT15) mouse monoclonal antibody, clone 6E7, Ascites
- 靶点：
KRT15
- 应用范围：
ELISA, FC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM06387SU-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态：
State: AscitesState: Ascitic fluid containing 0.03% sodium azide.
- 免疫原：
Purified recombinant fragment of KRT15 expressed in E. Coli.
- 规格：
100 ul
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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