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- 文献和实验
- 技术资料
- 抗体名:
ABC3735 goat polyclonal antibody, Biotin, Purified
- 抗体英文名:
ABC3735 goat polyclonal antibody, Biotin, Purified
- 应用范围:
ELISA, IP, WB
- 宿主:
Goat
- 适应物种:
Bacillus sp.
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
R1118BS
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 克隆性:
Polyclonal
- 保存条件:
Store lyophilized at 2-8°C for 6 months or at -20°C long term.After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Dilute only prior to immediate use.Avoid repeated freezing and thawing.
- 形态:
0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2Label: BiotinState: PurifiedState: Lyophilized purified Ig fractionStabilizer: 10 mg/ml BSA (immunoglobulin and protease free)Preservative: 0.01% Sodium azide
- 免疫原:
Uricase from Bacillus species
- 规格:
100 ug
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文献和实验The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The AvidinBiotin Complex (ABC) Method and Other AvidinBiotin Binding Methods
biotin complex (ABC) is then introduced to the biotinylated secondary antibody, where it binds to primary antibody-antigen sites. Also, enzyme-labeled avidin molecules can be used to bind biotinylated secondary antibodies with greater resolution. Finally
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