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- 文献和实验
- 技术资料
- 抗体名:
Cd28 hamster monoclonal antibody, clone 37.51, Purified
- 抗体英文名:
Cd28 hamster monoclonal antibody, clone 37.51, Purified
- 靶点:
Cd28
- 应用范围:
FC, FN, IP
- 宿主:
Hamster
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM08037PU-N
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态:
Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.State: PurifiedState: Liquid purified Ig fraction.
- 免疫原:
C57BL/6N mouse T cell lymphoma EL-4 cell line.
- 规格:
500 ug
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文献和实验Monoclonal Antibody Production
drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
分钟收集细胞; 5. 将上一步离心得到的细胞沉淀,重悬至扩增培养基中,并进行计数。 1.3 细胞刺激(次日) 扩增培养基成分:RPMI1640 + 10% 血清 + 5ug/ml anti-mouse CD28(克隆号:37.51) + 双抗 1. 将细胞浓度调整至 1-2×10^6/ml(本次实验为 2×10^6/ml,该浓度偏大); 2. 从 4°C 冰箱取出前一天包被的板子,弃掉包被液,无菌PBS洗涤3遍; 3. 每孔加入 500μl 细胞悬液,放置于 37°C 含 5% CO2 细胞
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