- ￥4724
- OriGene
- 美国
- AM32363PU-N
- 2026年04月01日
- IHC
- Mouse
- Human
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- 文献和实验
- 技术资料
- 免疫原：
In vitro activated B-cells.Source: A BALB/c mouse was immunized and SP2/0 cells were used for fusion with splenocytes.
- 形态：
PBSState: PurifiedState: Liquid purified Ig fractionPreservative: 0.05% sodium azide
- 保存条件：
Store the antibody undiluted at 2-8°C.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM32363PU-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
IHC
- 抗体英文名：
CDw78 mouse monoclonal antibody, clone AX15.3, Purified
- 抗体名：
CDw78 mouse monoclonal antibody, clone AX15.3, Purified
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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