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- 文献和实验
- 技术资料
- 免疫原:
Purified B cells from mouse lymph nodes.
- 形态:
PBS, pH 7.2State: PurifiedState: Liquid purified IgG fraction from Tissue Culture SupernatantStabilizer: 0.1% BSAPreservative: 0.09% Sodium Azide
- 保存条件:
Store undiluted at 2-8°C for up to one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
SM035P
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Rat
- 应用范围:
FC, IF, IHC, IP
- 靶点:
Ptprc
- 抗体英文名:
CD45 / LCA rat monoclonal antibody, clone IBL3/16, Purified
- 抗体名:
CD45 / LCA rat monoclonal antibody, clone IBL3/16, Purified
- 规格:
250 ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
7.16 anti-rat IgG2b). -------------------------------------------------------------------------------- 5) For full details see: Clark, M.R., & Waldmann, H. Production of murine monoclonal antibodies in Methods in Hematology 13:1-20 (1986
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
Immunofluorescent Staining of Mouse and Rat Leukocytes
(or 50 µl wash buffer for negative controls). Mix by gently vortexing or tapping. Incubate at 4°C for 20-40 min in the dark. Wash 2X with 200 µl wash buffer (or 3X if a biotin-conjugated primary antibody is used). After each centrifugation, 350 x g
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