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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Fusion protein comprising extracellular domain of human Fas linked to human Fc.Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
- 形态:
PBS, pH7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.Label: FITCState: Liquid purified IgG fraction.Label: Fluorescein Isothiocyanate Isomer 1
- 保存条件:
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
SM1203F
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
FAS
- 抗体英文名:
CD95 (FAS) mouse monoclonal antibody, clone LOB3/17, FITC
- 抗体名:
CD95 (FAS) mouse monoclonal antibody, clone LOB3/17, FITC
- 规格:
100 ug
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文献和实验an important role in regulation of the immune system. In the method described, hepatoma MH134 (Fas− and FasL− ) is transfected with murine Fas and FasL cDNA. A single administration of monoclonal anti-Fas antibody efficiently suppresses the growth of F6b (MH134
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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