- ￥5379
- OriGene
- 美国
- SM1222HRP
- 2026年04月01日
- IHC
- Mouse
- Bovine, Feline, Human, Monkey, Primate
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- 文献和实验
- 技术资料
- 免疫原：
Purified human tonsil lymphocyte membranes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
- 形态：
PBS, pH 7.4 containing 0.01% ThiomersalLabel: HRPState: Liquid purified IgG fractionLabel: Horseradish Peroxidase
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性：
Monoclonal
- 适应物种：
Bovine, Feline, Human, Monkey, Primate
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
SM1222HRP
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
IHC
- 抗体英文名：
HLA Class I ABC mouse monoclonal antibody, clone W6/32, HRP
- 抗体名：
HLA Class I ABC mouse monoclonal antibody, clone W6/32, HRP
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
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