3-Chloro-2-Methylacetanilide,7

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

1954, Pearson et al 1963). The most common such substrate is an indole derivative, 5-bromo-4-chloro-3-indolyl-ß-D-galactoside (X-gal, Holt and Sadler, 1958). When ß-gal cleaves the glycosidic linkage in X-gal, a soluble, colorless indoxyl monomer

Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection

(HPLC) (9 –32 ), GC-mass spectrometry (GC-MS) (33 ,34 ), liquid chromatography-mass spectrometry (LC-MS) (35 ), and capillary zone electrophoresis (CZE) (36 –38 ). However, isotachophoresis and AAA methods are nonselective for sulfur amino acids

Cell Staining for Senescence-Associated beta-Galactosidase (SA-beta-Gal) Activity

as before in PBS two times. 7. Add between 1 to 2 ml of Staining Solution per 35 mm culture dish or cover slide. 8. Incubate for at least 2 hr at 37°C (do not incubate in a CO2 incubator; see Hint #3). Recipes Staining Solution 1 ml of 100 mM K3Fe(CN