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- 文献和实验
- 技术资料
- 抗体名:
PE Mouse anti-human CD138 (Syndecan-1)(DL-101)
- 抗体英文名:
PE Mouse anti-human CD138 (Syndecan-1)(DL-101)
- 靶点:
-
- 浓度:
5μl/test
- 应用范围:
FC
- 宿主:
Mouse
- 适应物种:
-
- 保质期:
Store at 4°C.Do not freeze. Light sensitive material.
- 抗原来源:
-
- 目录编号:
-
- 级别:
-
- 库存:
现货
- 供应商:
爱必信(上海)生物科技有限公司
- 标记物:
-
- 克隆性:
-
- 保存条件:
Store at 4°C.Do not freeze. Light sensitive material.
- 形态:
液体
- 亚型:
-
- 免疫原:
-
- 规格:
25T/100T
| 规格: | 25T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 100T | 产品价格: | ¥3380.0 |
| 概述 | |
| 宿主 | Mouse |
| 反应种属 | Human |
| 应用 | FC |
| 性能 | |
| 形式 | Liquid |
| 浓度 | 5μl/test |
| 类型 | Monoclonal Antibody |
| 克隆号 | DL-101 |
| 同种型 | Mouse IgG1,κ |
| 储存/保存方法 | Store at 4°C.Do not freeze. Light sensitive material. |
| 存储溶液 | Phosphate-buffered solution, pH 7.2-7.4, containing 0.05% Proclin 300 and 1% FBS |
| 标记 | PE |
| 使用方法 | Excitation Laser:Blue 488 nm,Green 532 nm,Yellow/Green 561 nm Ex/Em Max:496nm/578nm |
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
protocol only addresses the use of mouse stem cells, this system has been successfully adapted for the generation of T-lineage cells from human HSCs (La Motte-Mohs et al. 2005 ; Awong et al. 2008 ). Although OP9 cells provide a robust stromal cell
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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