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- 供应商:
西宝生物
- 库存:
大量
- 规格:
1KG
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文献和实验Isolation of Bacterial Cell Membranes Proteins Using Carbonate Extraction
for solubilisation. Incubation of cell membrane preparations with sodium carbonate solution enriches for cell membrane proteins by stripping away other loosely associated protein contaminants from membranes (e.g., ribosomes, elongation factors). With the aid
0,02% sodium thiosulfate for 1 min.5)Rinse with water for 1 min.2X.0.02% Sodium Thiosulfate: For 1 gel,add 30mg sodium thiosulfate-5 hydrate to 150ml water.Silver reaction6)Submerge gel in 150ml 0.1% silver nitrate with 0.08% formalin (37%)for 20 min
Periodate Treatment of Fixed Cell Plates
1. Reagents Sodium Borohydride (NaBH4) - 1 mg/ml Carbonate:Bicarbonate Buffer, pH 9.6 Sodium Periodate (NaIO4) - 1 mM, 2.14 mg/10 ml Acetate Buffer 10mM Acetate Buffer, pH 4.5 0.82 g sodium acetate in 90 ml diH2O. Adjust to pH
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