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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
ROOM TEMPERATURE
- 英文名：
COLUMBIA AGAR BASE
- 库存：
500 g
- 供应商：
西宝生物
- 规格：
CELL BIOLOGY
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文献和实验SOFT AGAR ASSAY FOR COLONY FORMATION
Note: All volumes are calculated to cater for four plates per point. Base Agar 1. Melt 1% Agar (DNA grade) in microwave, cool to 40 in a waterbath. Warm 2X RPMI + 20% FCS to 40 in waterbath. Allow at least 30 minutes for temperature
Hard Agar Medium for Egg Collection Plates
This is a firm medium that keeps well and makes a good base for egg collecting. Wrap filled petri dishes and store at 4-- 14o C (they should keep for at least 6 weeks). Substitute molasses for the corn syrup if you prefer
. Procedure Prepare petri plates with 0.4 cm of base agar medium. LB (or other nutrient) with 1.2% agar for bacteria YPD (or other nutrient) with 2% agar for yeast Prepare 0.7% agar medium (soft agar
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